Science Heritage Journal | Galeri Warisan Sains (GWS)

THE EFFECT OF AQUEOUS EXTRACTS OF NIGELLA SATIVA ON BREAST CANCER CELL LINE MCF-7: AN IN VITRO STUDY

gws.01.2018.13.17

ABSTRACT

 

THE EFFECT OF AQUEOUS EXTRACTS OF NIGELLA SATIVA ON BREAST CANCER CELL LINE MCF-7: AN IN VITRO STUDY

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Muhammad Solehuddin Bumidin, Farah Azurin Johari, Nurul Fikri Risan, Mohd Hamzah Mohd Nasir

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2018.13.17

Nigella sativa (NS) or also known as black seeds is one of the traditional herbs that were identified to have anticancer properties due to the presence of an active compound, thymoquinone. In this study, the cytotoxic effect of the aqueous extracts of NS were tested on the breast cancer cell line, MCF-7. Aqueous extraction of NS using cold water producing 3.8% of yield. The cytotoxic effect of aqueous extracts of NS were tested after incubation at 5 hours, 24 hours and 48 hours by using a modified MTT colorimetric assay. Interestingly, the IC50 of aqueous extracts of NS on MCF-7 was at 11.5 µg/ml. Additionally, aqueous extracts of NS at the concentration beyond 11.5 µg/ml causing the swelling of MCF-7 and leads to cell burst. In conclusion, aqueous extracts of NS have been proved to have cytotoxic effect on MCF-7.

Pages 13-17
Year 2018
Issue 1
Volume 2

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gws.01.2018.10.12

ABSTRACT

 

PHYSICOCHEMICAL PARAMETERS AND BACTERIAL COMPOSITION IN SUNGAI PUSU GOMBAK

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Noor Faizul Hadry Nordin, Nur Hazirah Syaza binti Ibrahim, Ahmed Jalal Khan Chowdhury

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2018.10.12

Sungai Pusu is a river that flows through the campus of the International Islamic University Malaysia (IIUM), Gombak. This river could have been affected by pollution and urbanization making the water to be cloudy in appearance. The massive deterioration of the river was identified caused by housing construction at the upstream. There is also waste dumping area located near tributary of Sungai Pusu which may lead to leachate seeping into the river. The location within university area with lots of residents makes the river exposed to daily pollution. This study was carried out with samples taken from 8 stations within the campus to determine the bacterial distribution and the physicochemical parameters at the Sungai Pusu, Gombak. Physicochemical parameters (temperature, specific conductivity, dissolved oxygen and pH) were analyzed by using Hydrolab (Data Sonde 4A). Temperature range was found from 30°C to 34°C, pH range from 7.67 to 8.42, dissolved oxygen ranged from 2.80mg/l to 4.88mg/l, and specific conductance ranged from 0.041ms/cm to 0.134ms/cm. High bacterial colony (cfu mL-1) was observed at Station 8 (39,272 cfu mL-1) followed by Station 5 (30,311 cfu mL-1) while, the lowest bacterial colony was found at Station 6 (2,005 cfu mL-1). This study provides an overview on the distribution of the bacterial varied with physicochemical parameters throughout the river which might be due to the human intervention and natural.

Pages 10-12
Year 2018
Issue 1
Volume 2

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gws.01.2018.04.09

ABSTRACT

DIVERSITY AND DISTRIBUTION OF FERN SPECIES IN SELECTED TRAIL IN KUANTAN PAHANG

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Izyan Nazihah, Mohd Shahir Zaini, Rozilawati Shahari, Che Nurul Aini Che Amri, Nurshuhada Mohammad Tajuddin

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2018.04.09

A study on the diversity and distribution of terrestrial and epiphytic fern in Kuantan, Pahang was conducted from January to November 2017. Fourteen species of both terrestrial and epiphytic ferns, respectively have been collected and examined from five districts of Kuantan namely Kuala Kuantan, Ulu Kuantan, Penor, Ulu Lepar and Sungai Karang. This study was carried out to examine and evaluate the distribution and to assess the abudance of epiphytic fern in Kuantan, Pahang. The materials obtained for this study were collected from sampling sites and prepared as herbarium voucher. Morphological characteristics were studied, observed and annotated. Based on this study, the terrestrial and epiphytic fern families namely Nephrolepidaceae, Gleicheniaceae, Lygodiaceae, Lindsaeceae, Woodiaceae and Thelypteridaceae, Polypodiaceae, Pteridaceae, Davalliaceae, Aspleniaceae, Blechnaceae and Cyatheaceae were recorded. It mostly can be found in the rainforest area. An updated checklist of terrestrial and epiphytic ferns has been prepared for comparison and future reference.

Pages 04-09
Year 2018
Issue 1
Volume 2

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gws.01.2018.01.03

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ANTIMICROBIAL EFFICACY OF TAMARIX DIOCA (L.) LEAVES AND FLOWERS

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Abdurahim Khan, Muhammad Fiaz, Rahmat Ali khan, Jan Baz Khan, Farhad Ullah khan, Zain ul Wahab

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2018.01.03

Tamarix dioca (L.) belongs to family Tamaricaceae is traditionally a vital plant used for treatment of various diseases. Efforts were made to investigate the antimicrobial efficiency of T. dioca.There was three concentrations of crude methanolic extracts of 200μg/ml, 100μg/ml and 50 μg/ml were checked against six pathogenic fungi Aspergillus fumigatus, aspergillus flavus, Fusarium solani, Aspergillus niger, Penicillium digitatum and Penicillium tubesulum and six bacterial strains Bacillus subtilis, Enterobactor aerogenes, Escherichia coli, Kliebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus. It was noted that Percentage inhibition in the growth of fungi and bacteria was dosage dependent. Terbinafine a standard antifungal drug, 10mg/ml and Cefexime 10mg/ml (antibacterial) were used as a positive control. The results were compared with control and most of the results were found significant. Maximum inhibition was showed by T. dioca against fungal strain A. niger (74%) and bacterial strain K. pneumoniae (48%). It can be use as powerful antimicrobial agent in near fture.

Pages 01-03
Year 2018
Issue 1
Volume 2

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gws.02.2017.32.33

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Noise exposure during orthopaedics surgery

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Ailin Razali, Hendy Putra Herman, Ahmad Hafiz Zulkifly

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2017.32.33

There is serious concern on noise hazard in operating theatre especially in the orthopaedics field that requires usage of many instruments. However, data on this noise exposure particularly in Malaysia is rather limited. This study was conducted as a pilot study to investigate the noise exposure in orthopaedics theatre in a hospital in Kuantan, Pahang in June 2011. Several different orthopaedics theatres involving procedures that use powered instruments were investigated. Time recorded noise exposures were taken using personal noise dosimeters throughout the surgeries worn by the surgeons. The peak noise level and the average noise exposure within 8 hours were determined, and compared with the available Factory and Machinery Act (Noise) standards. Six out of seven surgeries exceeded 140 dBA for peak sound levels. The average noise exposure within 8 hours was below 85 dBA in all cases. Although the average noise level did not surpass the stipulated guidelines, the peak level exceeding 140 dBA still carries risk for hearing loss. In conclusion, healthcare personnel in operating theatre were exposed to noise hazard for certain medical procedures. Hence, proper noise management should be implemented to protect them from noise-induced hearing loss while maintaining the success and efficiency of the surgeries.

Pages 32-33
Year 2017
Issue 2
Volume 1

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gws.02.2017.29.31

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Study of cucumber mosaic virus gene expression in capsicum annuum

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Nur Hafizah Azizan, Zulkifli Ahmad Zainal Abidin, Ing Chia Phang

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2017.29.31

Cucumber mosaic virus (CMV) is a plant pathogenic virus in the genus Cucumovirus, family Bromoviridae. It has the potential and reputation of having the widest host range of any known plant virus including monocotyledons and dicotyledons, herbaceous plants, shrubs and trees. CMV is one of the major diseases in Capsicum annum (chilli). Chilli plant samples exhibiting virus-like disease symptoms were collected from Taman Pertanian Indera Mahkota (location 1) and Greenhouse 12 of Horticulture Research Centre, Serdang (location 2). Viral disease was detected based on symptoms like mosaic-mottling, yellow ringspots and cholorotic that appeared on the leaves. The isolation of total RNA was done by using Vivantis GF-1 total RNA extraction kit. RT-PCR technique was used to detect the presence of virus disease symptoms gene in chillies. Identification of causal agents was based on cDNA amplified product size, using virus-specific oligonucleotides. Actin was used as the internal PCR control. The product size of the DNA fragment was 315 bp. From RT-PCR, the expression of CMV can be detected in chilli plants that exhibited the virus-like disease symptoms. This research revealed that some of the chilli plants at the Taman Pertanian Indera Mahkota and Greenhouse 12 of Horticulture Research Centre have been affected by this viral disease.

Pages 29-31
Year 2017
Issue 2
Volume 1

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gws.02.2017.23.28

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The investigation of cytotoxic effect of cinnamomum zeylanicum extracts on human breast cancer cell line (mcf-7)

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Widya Abd Wahab, Ainatun Natrah Adzmi

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2017.23.28

Cinnamon is one of the oldest herbal medicine that has been historically known to possess has anticancer property. In this study, the cytotoxicity of the cinnamon extracts towards human breast cancer cell line (MCF-7) was investigated. Cinnamomum zeylanicum was extracted using Soxhlet and water extraction methods, producing 7.06% and 2.54% of yield respectively. The extracts were analysed using Gas Chromatography/ Mass Spectrometry (GCMS) and it was found that cinnamaldehyde is the major constituents which contribute more than 70% of the major constituents. The cytotoxicity of C. zeylanicum extract against MCF-7 was determined through colorimetric MTT assay at 24 and 48 hours of incubation periods. The IC50 values of Soxhlet extract were 58 µg/ml and 140 µg/ml while the IC50 values of water extract were 9 mg/ml and 4.8 mg/ml, at 24 and 48 hours treatment respectively. In conclusion, C. zeylanicum extracts has been confirmed to have cytotoxic effect towards MCF-7 cell line in different concentrations at two different incubation periods.

Pages 23-28
Year 2017
Issue 2
Volume 1

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gws.02.2017.19.22

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Drag reduction for a fast back passenger car (logan)

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Suliman Mohammed Mohammed Ali, Jalal Mohammed Zayan

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2017.19.22

The car manufacturers all over the globe are intensively competing among each other in terms of the design and fuel consumption factor. Nowadays the reduction of drag is becoming a very important challenge for all the car manufacturers as they are producing powerful cars with better fuel consumption in the market regulated with law reinforcement on fuel emissions and consumers. Lower drag provides better performances such as higher top speed and better stability. It also often lowers aerodynamic noise and greenhouse gas emission above all decreases in fuel consumption. However, modern designs of cars tend to go higher and wider and thus they have higher frontal areas due to the functional, economic and aesthetic requirements. Increasing frontal area of the vehicle tend to increase the drag force acting on the vehicle which is proportional to the dimensionless drag coefficient and the projected area of the vehicle. Consequently, to hold or even decrease the drag on a car that has a larger frontal area, tremendous effort has to be made. In this research both experimental and numerical analysis have been done to an existing model of a car with some various aerodynamic add-on devices that can be attached to car and reduce aerodynamic drag of the vehicle without comprising on its main design features.

Pages 19-22
Year 2017
Issue 2
Volume 1

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gws.02.2017.16.18

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Cytotoxicity on mcf7 cell lines exposed to an extract of the jacalin from jackfruit seed

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: M. A Zuraidah, B. Akbar John, Y. Kamaruzzaman

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2017.16.18

Jacalin is a major lectin present in jackfruit seeds, obtained by crude protein recovery. Its lectin symbol is AIL and it belongs to the galactose family of N-acetylglucosamine binding lectins, which are recognized to be cancer cell inhibitor. There have been many pharmacological research studies focusing intensively on jacalin, however their scope was restricted to the application of jacalin in pharmacology. Jacalin or lectin extract from jackfruit seed were induced cell cycle arrest in human breast cancer cell, MCF7, in comparison of crude extract, purified jacalin and jacalin standard. IC50 for MCF7 was achieved at concentration 125 µg/mL, comparable to jacalin standard with only 1.60 % contradictions.

Pages 16-18
Year 2017
Issue 2
Volume 1

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gws.02.2017.11.15

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Isolation and characterization of biosurfactant producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Noor Shaidatul Lyana Mohamad Zainal, Suhaila Mohd Omar, Mardiana Mohd Ashaari

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2017.11.15

Surfactants have been widely used to facilitate biodegradation of petroleum pollutant; particularly those are being produced by microorganisms which are also known as biosurfactant. Six bacterial isolates from petroleum contaminated sites were found to be potential hydrocarbon degrader candidates based on their ability to grow on minimal media supplemented with petroleum crude oil as sole carbon source. In addition, they also found to be having potential in producing extracellular biosurfactant based on the results of screening for biosurfactant activity. These isolates were further identified and characterized. Morphological and biochemical characteristics of these isolates have been examined and molecular identification was done by amplifying 16S rRNA gene. Three isolates were identified as the member of the genus Pseudomonas; another two isolates were the member of genus Comamonas and one isolate from the genus Stenotrophomonas. Several qualitative screening methods (microplate assay, oil displacement test, emulsification assay and drop-collapse test) showed variation of results; suggesting the need to support these findings with quantitative screening based on measurement of surface activity. BSP 6 which was found to be closely related to Comamonas aquatica was the most promising biosurfactant producer found in this study based on two positive results out of four qualitative screening methods performed.

Pages 11-15
Year 2017
Issue 2
Volume 1

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