In order to forecast the effects of the fungi on humans, this study was conducted to isolate and identify the fungi in three distinct samples in the college of science cafeteria. In order to obtain well-isolated colonies, the power plate method with Potato Dextrose Agar (PDA) was used to isolate and identify various fungal species. The results show the identity and the total colony forming units (CFU) for fungi. The most frequently isolated fungi were Cladosporium, Rhizopus sp., Aspergillus Sp, Alternaria, Yeast, Penicillium, . While the less frequently detected fungi species were Trichocladium, Drecheslera sp.and Botrytis sp. According to the fungi the higher numbers of fungi were isolated on menu and containers.