An 8-week feeding trial was conducted to investigate the effect of feeding time on growth, morphology, and amino acid composition in juvenile African catfish (Clarias gariepinus) in a concrete fish pond. Fish (6.03 ± 0.16 g) was divided into four groups at a stocking density of 10 per pond with different feeding time intervals: group (A) (control) fed 08:00, 13:00, 20:00 h), Group B (08:00 and 13:00), Group C (13:00 and 20:00), and Group D (08:00 and 20:00) in three replicate for 8 weeks. According to the result, Groups B and C obtained body mass index (BMI), and daily growth rate (DGR), lipid gain, and organ indices compared to the control. In addition, whole-body and muscle crude protein were reduced, with an opposite trend observed in nitrogen-free extract in Groups B and C, while Group D was almost similar to the control. This was also similar for the levels of the essential amino acids (threonine, lysine, methionine and phenylalanine) and non-essential amino acids related (serine, glycine) profile. Overall, the findings suggest that timing feeding with the endogenous rhythms of C. gariepinus enhances growth, nutrient utilization and health, possibly yielding an economically viable aquaculture production.