Sacred Natural Sites (SNSs) have been traditionally associated with health benefits across cultures. This study investigated the physiological effects of SNSs visits using biofield analysis.The Gas Discharge Visualization (GDV) technique was used to do 40 measurements on 13 sites in Burgundy, France. Pre- and post-visit measurements assessed physiological stress, autonomic nervous system balance and homeostasis. Control measurements were taken at ordinary natural sites.Significant improvements were observed in three key parameters: physiological stress reduction (-12%, p=0.002), improved autonomic nervous system balance (-26% of organs out of balance, p 0.001), and enhanced homeostasis (-20% of organs out of norm, p=0.009). Gender differences were noted, with men showing more pronounced responses. Sacred Natural Sites demonstrate measurable harmonizing effects on human physiology, distinct from ordinary natural environments. These findings support traditional knowledge about SNSs health benefits and suggest potential applications in preventive healthcare and site conservation strategies.