Science Heritage Journal | Galeri Warisan Sains (GWS)

ANTIOXIDANT AND CYTOTOXIC EFFECTS OF CURCUMA MANGGA AND BOSENBERGIA ROTUNDA ETHANOLIC EXTRACTS ON MCF-7 CANCER CELL LINES

Author archives:

gws.02.2019.10.14

ABSTRACT

ANTIOXIDANT AND CYTOTOXIC EFFECTS OF CURCUMA MANGGA AND BOSENBERGIA ROTUNDA ETHANOLIC EXTRACTS ON MCF-7 CANCER CELL LINES

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Tamil C.M. Sundram, Mohd Hazim Bin Zakaria and Mohd Hamzah Bin Mohd Nasir

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2019.10.14

Curcuma mangga and Bosenbergia rotunda which belongs to Zingiberaceae family are largely distributed in South East Asian countries including Malaysia. These plants have been traditionally used for treatment of various diseases. In this research we focused on determining the antioxidant and cytotoxic activity of C. mangga and B. rotunda ethanolic extracts. Initially, in this study, ethanolic extracts from rhizomes of C. mangga and B. rotunda were obtained through Soxhlet extraction method. 12.6% and 3.5% of crude extract were obtained from C. mangga and B. rotunda respectively. For MTT assay, the IC50 values are 207µg/ml and 31.96µg/ml for C. mangga and B. rotunda respectively. Meanwhile for the antioxidant activity using DPPH assay, C. mangga showed higher antioxidant activity with IC50 values of 1073µg/ml compared to B. rotunda with IC50 values of 578µg/ml. It can be concluded both extracts showed low inhibitory effect on MCF-7 cancer cells growth and DPPH free radical activity.

Pages 10-14
Year 2019
Issue 2
Volume 3

Download

Posted by Nurul

gws.01.2020.01.02

ABSTRACT

CALLUS INDUCTION FROM LEAF EXPLANT OF FICUS DELTOIDEA VARKUNSTLERI

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Hafizuddin Sa’adan, Zarina Zainuddin

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2020.01.02

Ficus deltoidea or commonly known as ‘mas cotek’ is a herbal plant indigenous to Southeast Asia including Malaysia and Indonesia. This plant is popular for its medicinal values such as improve blood circulation, regain energy and enhance fertility naturally for both men and women. The main objective of this study is to develop in vitro clonal propagation method for rapid production of F. deltoidea using different concentrations of benzyl aminopurine (BAP) through shoot induction and multiplication, rooting and subsequent establishment in soil following acclimatization. Surface sterilization of the leaf explants was done using mercury chloride and ethanol as the disinfectants. Pre-treatment of the explants with carbendazim successfully reduced the occurrence of fungal contamination. At the end of the experiment, no shoot and root induction were observed but calli were successfully induced on MS medium containing 1.0, 1.5, 2.0, 2.5 and 3.0 mg/l BAP, with calli induced from 3.0 mg/l BAP were bigger and healthier. In short, the higher the concentration of BAP used, the higher tendency for the explant to induce callus.

Pages 01-02
Year 2020
Issue 1
Volume 4

Download

Posted by Nurul

gws.01.2019.17.19

ABSTRACT

ANTI-DIABETIC POTENTIAL OF PEPTIDE FROM P. NIRURI REVEALS THROUGH CARBOHYDRATE HYDROLYZING ENZYME INHIBITION ASSAY

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Noor Hasniza Md Zin, Ainul Mardhiah Mohd Nail

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2019.17.19

Herb Phyllanthus niruri (P. niruri) is known to have various pharmacological functions including anti-diabetic properties. In this research, protein extracts from different plant parts (leave, fruit and stem) of P. niruri were investigated for their anti-diabetic potential through α-amylase and α-glucosidase enzyme inhibition assays. For the enzyme inhibition assay, fruit was found to have the highest inhibition percentage (90.0%) against α-glucosidase followed by leave (62.6%) and stem (38.4%). The similar patterns were also recorded for the α-amylase enzyme inhibition assay, in which, fruit showed the highest inhibition percentage (64.1%) followed by leave (33.3%) and stem (18.2%). The findings of this research suggest that fruit of P. niruri is a potential plant part with regards to anti-diabetic properties since it exhibits the highest inhibition activity against both α-amylase and α-glucosidase enzyme compared to leave and stem.

Pages 17-19
Year 2019
Issue 1
Volume 3

Download

Posted by Nurul

gws.01.2019.11.16

ABSTRACT

PROTEOMICS AS TOOLS FOR BIOMARKERS DISCOVERY OF ADULTERATION IN SLAUGHTERING PROCEDURES

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Azura Amid, Norshahida A. Samah

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2019.11.16

Slaughtering is an unavoidably sensitive issue among Muslim and Jews. This paper gives an outlook on possible detection methods in resolving the dilemma of religious slaughtering status. It will be rather easier to differentiate meat of different animal origins due to the exclusive genetic blueprint. However, in the case of adulteration in slaughtering procedure, the meat was taken from a similar source, thus, complicating the detection process. Therefore, an alternative approach employing proteomics were developed to identify protein expression patterns after external stimulation with electrical treatment. In the slaughtering process, the pain which is triggered by an external stimulus is expected to influence the protein profiles. Therefore, variations in stunning treatments which result in different patterns of protein profiles will pinpoint the specific biomarker for over stunned animals. This will inevitably help to detect adulterations in slaughtering procedure.

Pages 11-16
Year 2019
Issue 1
Volume 3

Download

Posted by Nurul

gws.01.2019.08.10

ABSTRACT

IN-VITRO REGENERATION OF ORTHOSIPHON STAMINEUS (MISAI KUCING) USING AXILLARY BUD

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Zarina Zainuddin, Asmaa’ Mohd Kamil

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2019.08.10

Orthosiphon stamineus or known as Misai kucing is a popular herbal tea plant that helps in treating the ailments of kidney and bladder, diabetes mellitus and gout. Due to high demand of this medicinal plant, a large propagation of this plant is recommended. In this study, a protocol for regeneration of Orthosiphon stamineus using axillary bud as explant was established. Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) were evaluated for their effects on shoot induction. Results obtained revealed that the best shooting ability was observed when explants were cultured on MS medium supplemented with 0.2 ppm of BAP. Subsequently, shoot elongation of established shoot was obtained in medium containing gibberelic acid (GA3) with concentration of 0.5 ppm and 1.0 ppm. The best shoot elongation was achieved with medium supplemented with 0.5 ppm of GA3. Subsequently, the elongated explants were transformed to root induction medium with the addition of either 0.2 ppm of 1-naphthaleneacetic acid (NAA) or 0.5 ppm NAA. The explants showed positive response in medium supplemented with 0.2 ppm of NAA. From this study it could be confirmed that suitable concentration of plant growth regulators could be used in the regeneration of Orthosiphon stamineus through tissue culture technique.

Pages 08-10
Year 2019
Issue 1
Volume 3

Download

Posted by Nurul

gws.01.2019.01.07

ABSTRACT

ANTIOXIDANT CAPACITY OF THE GREEN LEAFY VEGETABLES USING OXYGEN RADICAL ANTIOXIDANT CAPACITY (ORAC), 2,2′-AZINO-BIS (3-ETHYLBENZOTHIAZOLINE-6-SULPHONIC ACID(ABTS) AND 2,2-DIPHENYL-1-PICRYLHYDRAZYL (DPPH) ASSAYS

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Normah, H, Hanapi, M. J.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2019.01.07

Antioxidants are believed to play a very important role in the body defence system against reactive oxygen species (ROS), the harmful by-products that are generated during normal aerobic cell respiration. The objective of this study was to determine the antioxidant capacity in green leafy vegetables using ORAC, ABTS, and DPPH assays of different polyphenol fractions (free phenolic, alkaline hydrolysate, acidic hydrolysate). The antioxidant capacity of the identified free and bound phenolic acid content was measured using different assays including ORAC, ABTS, and DPPH assay (end-point assay and kinetic assay). Only hydrophilic antioxidant activities of all selected samples were examined using ORAC assay. Strong correlations were observed in acidic and alkaline hydrolysate fractions (p<0.01) as determined by ORAC and ABTS assays, respectively. In the free phenolic acid extracts, the O. basilicum (Sweet basil) ranked first, had highest antioxidant capacities of 521804±4243 µmol TE/100g DW, 329.8±0.4mg TE/g DW and 9.0±1.8 µg GAE/g DW as determined by ORAC, ABTS and DPPH, respectively. . The A. occidentale (Cashew shoot) in the alkaline hydrolysate extract showed a greatest antioxidant capacity in all three assays: 889126±7193 µmol TE/100g DW, 466.5±7.9 mg TE/g DW and 3.5±0.4 µg GAE/g DW as measured by ORAC, ABTS and DPPH, separately. While, in acidic hydrolysate, the A. occidentale (Cashew shoot) extract also dominated the antioxidant capacity with (560504±5785 µmol TE/100g DW, 387±0.7 mg TE/g DW and 5.9±0.5 µg GAE/g DW) as determined by ORAC, ABTS and DPPH assays, respectively. The acidic and basic hydrolysis yielded higher antioxidant capacities in the present study. It suggests that hydrolysis with alkaline and acidic play significant roles in liberating more phenolic acids and flavonoids and generating high antioxidant capacity in the extracts.

Pages 01-07
Year 2019
Issue 1
Volume 3

Download

Posted by Nurul

gws.02.2018.27.29

ABSTRACT

ALPHA-AMYLASE INHIBITORY ACTIVITY OF INHIBITOR PROTEINS IN DIFFERENT TYPES OF COMMERCIAL RICE

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Nur Syazila Ramli, Noor Hasniza Md Zin

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2018.27.29

The inhibition of α-amylase enzyme by the inhibitor proteins extracts of all the three types of commercial rice, white rice (WR), brown rice (BR) and glutinous rice (GR) were investigated. Among the three types of rice, the BR has significantly the highest concentration of the inhibitor protein (0.030 ± 0.002 mg/ml) compared to glutinous rice (0.006 ± 0.001 mg/ml) and white rice (0.005 ± 0.001 mg/ml). In term of the percentage of inhibition of the α-amylase enzyme of the Aspergillus oryzae, the brown rice shows the highest inhibition (61.22 %) among the three types of rice with the lowest maltose liberated. The acarbose (0.1 mg/ml) which was used as a positive control represent the highest inhibition of the α-amylase enzyme inhibitor among all of the tested samples. Even though inhibitor protein extract of BR is possessed lower ability to inhibit the α-amylase, yet it still can be one of the best option and alternative for the Diabetes Mellitus patients for their daily consumption compared to the WR and GR.

Pages 27-29
Year 2018
Issue 2
Volume 2

Download

Posted by Nurul

gws.02.2018.21.26

ABSTRACT

ISOLATION AND SCREENING OF BIOSURFACTANT-PRODUCING MARINE
BACTERIA FROM KUANTAN PORT, PAHANG, MALAYSIA

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Muhammad Isminhaziq Ismail, Nur Hafizah Azizan, Mardiana Mohd Ashaari

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2018.21.26

Biosurfactants play an important role in bioremediation of organic pollutants such as petroleum hydrocarbon. The unique properties of biosurfactants make them possible to be used in the remediation of hydrocarbon contaminated sites. Therefore, the existence of indigenous microorganisms that have the ability to consume petroleum hydrocarbon as carbon source and simultaneously produce biosurfactants in order to facilitate the hydrocarbon metabolism can be manipulated for bioremediation purposes. In this study, isolation and screening of potential biosurfactant-producing bacteria from two sampling points in Kuantan Port seawater were successfully done. Amongst the isolates, 4 out of 7 isolates from Point A were Gram negative bacteria and 2 out 5 isolates from Point B were Gram negative bacteria. The positive oxidase test resulted for all isolates from Point A and only B5 from Point B produced negative result. Catalase test conducted produced positive results on isolates from Point A (A3, A5, A6& A7) and Point B (B1, B2, B4 & B5).The highest percentage emulsification index measured belonged to isolate B4 and B5 which are 67%, thus make these isolates to be the most promising biosurfactant producers. Further identification by 16S rRNA gene found that isolates were closely related to Rhodococcus erythropolis (A1), Psedomonas stutzeri (A2), Pseudoalteromonas lipolytica (A3, A6 and B4), Vibrio brasiliensis (A4 and B2), Vibrio tubiashii (B1), Marinobacter salsuginis (A5), Labrenzia aggregate (A7), Marinococcus halophilus (B3) and Thalassospira xianmenensis(B5). Hence, through biosurfactant activities exhibited by isolates, B4 and B5 were the most potential isolates to produce biosurfactant. Therefore, these isolates can potentially be exploited to aid in bioremediation of petroleum hydrocarbon contaminated sites and would also be useful to enhance oil recovery in petroleum industry.

Pages 21-26
Year 2018
Issue 2
Volume 2

Download

Posted by Nurul

gws.02.2018.16.20

ABSTRACT

RESEARCH ON MENTAL HEALTH STATUS AND THE RELATIONSHIP BETWEEN SPIRITUAL BELIEF AND SELF – HARMONY

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Liu Chuanlei , Li Guomin , Han Yuanfei, Wu Guojun

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2018.16.20

According to the questionnaire survey of 500 graduate students on mental health, spiritual belief and self-harmony, through mathematical statistical analysis, it was found that :(1) overall, the psychological status of graduate students was unhealthy, and there were significant differences in some demographic variables; (2) self-flexibility has a significant positive predictive effect on political belief, nationalism, life pursuit and family pursuit; (3) the rigidity of ego has significant negative and positive predictive effect on nationalism and money pursuit respectively; (4) the disharmony between self and experience has a significant positive predictive effect on religious belief and god worship.

Pages 16-20
Year 2018
Issue 2
Volume 2

Download

Posted by Nurul

gws.02.2018.10.15

ABSTRACT

CYTOTOXIC EFFECTS AND RESPONSE SURFACE OPTIMIZATION OF SOLVENT EXTRACTION OF CRUDE EXTRACTS FROM Aquilaria subintegra UNINFECTED BRANCH

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Phirdaous Abbas, Yumi Z.H-Y Hashim, Hamzah Mohd Salleh

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2018.10.15

Agarwood plant has been used in traditional medicine to treat wide range of disorders aside from the main function of its fragrant wood in perfumery. In this present study, we are interested to maximize the yield of extract and investigate the cytotoxic/anti-cancer effects of uninfected branch extracts. Uninfected branch is readily available from agarwood plantation particularly during the early years before inoculation process that formed the fragrant wood (resin); and as such is seen as an economical raw material. Uninfected branch from Aquilaria subintegra was subjected to response surface methodology (RSM) – guided ethanolic extraction to achieve maximum yield while maintaining the biological activity. Cytotoxicity/anti-cancer assays including cell attachment assay, cell viability assay and sulforhodamine B (SRB) assay were carried out on the extracts. Run 11 with 12 hours extraction time, 50 °C temperature, 100 rpm and 60 ml extraction volume gave the highest yield of 0.2130 ± 0.036 g/g agarwood branch extract (ABE). Experimental extract from run 16 showed the most promising cytotoxic effects against MCF-7 with IC50 of 8 µg/ml. The study showed that ABE possess potential cytotoxic/anti-cancer activities against MCF-7 cells and further research is warranted to identify the bioactive compounds and mechanism of action.

Pages 10-15
Year 2018
Issue 2
Volume 2

Download

Posted by Nurul