Science Heritage Journal | Galeri Warisan Sains (GWS)

ANTI-DIABETIC POTENTIAL OF PEPTIDE FROM P. NIRURI REVEALS THROUGH CARBOHYDRATE HYDROLYZING ENZYME INHIBITION ASSAY

Author archives:

gws.01.2019.17.19

ABSTRACT

ANTI-DIABETIC POTENTIAL OF PEPTIDE FROM P. NIRURI REVEALS THROUGH CARBOHYDRATE HYDROLYZING ENZYME INHIBITION ASSAY

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Noor Hasniza Md Zin, Ainul Mardhiah Mohd Nail

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2019.17.19

Herb Phyllanthus niruri (P. niruri) is known to have various pharmacological functions including anti-diabetic properties. In this research, protein extracts from different plant parts (leave, fruit and stem) of P. niruri were investigated for their anti-diabetic potential through α-amylase and α-glucosidase enzyme inhibition assays. For the enzyme inhibition assay, fruit was found to have the highest inhibition percentage (90.0%) against α-glucosidase followed by leave (62.6%) and stem (38.4%). The similar patterns were also recorded for the α-amylase enzyme inhibition assay, in which, fruit showed the highest inhibition percentage (64.1%) followed by leave (33.3%) and stem (18.2%). The findings of this research suggest that fruit of P. niruri is a potential plant part with regards to anti-diabetic properties since it exhibits the highest inhibition activity against both α-amylase and α-glucosidase enzyme compared to leave and stem.

Pages 17-19
Year 2019
Issue 1
Volume 3

Download

Posted by Nurul

gws.01.2019.11.16

ABSTRACT

PROTEOMICS AS TOOLS FOR BIOMARKERS DISCOVERY OF ADULTERATION IN SLAUGHTERING PROCEDURES

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Azura Amid, Norshahida A. Samah

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2019.11.16

Slaughtering is an unavoidably sensitive issue among Muslim and Jews. This paper gives an outlook on possible detection methods in resolving the dilemma of religious slaughtering status. It will be rather easier to differentiate meat of different animal origins due to the exclusive genetic blueprint. However, in the case of adulteration in slaughtering procedure, the meat was taken from a similar source, thus, complicating the detection process. Therefore, an alternative approach employing proteomics were developed to identify protein expression patterns after external stimulation with electrical treatment. In the slaughtering process, the pain which is triggered by an external stimulus is expected to influence the protein profiles. Therefore, variations in stunning treatments which result in different patterns of protein profiles will pinpoint the specific biomarker for over stunned animals. This will inevitably help to detect adulterations in slaughtering procedure.

Pages 11-16
Year 2019
Issue 1
Volume 3

Download

Posted by Nurul

gws.01.2019.08.10

ABSTRACT

IN-VITRO REGENERATION OF ORTHOSIPHON STAMINEUS (MISAI KUCING) USING AXILLARY BUD

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Zarina Zainuddin, Asmaa’ Mohd Kamil

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2019.08.10

Orthosiphon stamineus or known as Misai kucing is a popular herbal tea plant that helps in treating the ailments of kidney and bladder, diabetes mellitus and gout. Due to high demand of this medicinal plant, a large propagation of this plant is recommended. In this study, a protocol for regeneration of Orthosiphon stamineus using axillary bud as explant was established. Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) were evaluated for their effects on shoot induction. Results obtained revealed that the best shooting ability was observed when explants were cultured on MS medium supplemented with 0.2 ppm of BAP. Subsequently, shoot elongation of established shoot was obtained in medium containing gibberelic acid (GA3) with concentration of 0.5 ppm and 1.0 ppm. The best shoot elongation was achieved with medium supplemented with 0.5 ppm of GA3. Subsequently, the elongated explants were transformed to root induction medium with the addition of either 0.2 ppm of 1-naphthaleneacetic acid (NAA) or 0.5 ppm NAA. The explants showed positive response in medium supplemented with 0.2 ppm of NAA. From this study it could be confirmed that suitable concentration of plant growth regulators could be used in the regeneration of Orthosiphon stamineus through tissue culture technique.

Pages 08-10
Year 2019
Issue 1
Volume 3

Download

Posted by Nurul

gws.01.2019.01.07

ABSTRACT

ANTIOXIDANT CAPACITY OF THE GREEN LEAFY VEGETABLES USING OXYGEN RADICAL ANTIOXIDANT CAPACITY (ORAC), 2,2′-AZINO-BIS (3-ETHYLBENZOTHIAZOLINE-6-SULPHONIC ACID(ABTS) AND 2,2-DIPHENYL-1-PICRYLHYDRAZYL (DPPH) ASSAYS

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Normah, H, Hanapi, M. J.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.01.2019.01.07

Antioxidants are believed to play a very important role in the body defence system against reactive oxygen species (ROS), the harmful by-products that are generated during normal aerobic cell respiration. The objective of this study was to determine the antioxidant capacity in green leafy vegetables using ORAC, ABTS, and DPPH assays of different polyphenol fractions (free phenolic, alkaline hydrolysate, acidic hydrolysate). The antioxidant capacity of the identified free and bound phenolic acid content was measured using different assays including ORAC, ABTS, and DPPH assay (end-point assay and kinetic assay). Only hydrophilic antioxidant activities of all selected samples were examined using ORAC assay. Strong correlations were observed in acidic and alkaline hydrolysate fractions (p<0.01) as determined by ORAC and ABTS assays, respectively. In the free phenolic acid extracts, the O. basilicum (Sweet basil) ranked first, had highest antioxidant capacities of 521804±4243 µmol TE/100g DW, 329.8±0.4mg TE/g DW and 9.0±1.8 µg GAE/g DW as determined by ORAC, ABTS and DPPH, respectively. . The A. occidentale (Cashew shoot) in the alkaline hydrolysate extract showed a greatest antioxidant capacity in all three assays: 889126±7193 µmol TE/100g DW, 466.5±7.9 mg TE/g DW and 3.5±0.4 µg GAE/g DW as measured by ORAC, ABTS and DPPH, separately. While, in acidic hydrolysate, the A. occidentale (Cashew shoot) extract also dominated the antioxidant capacity with (560504±5785 µmol TE/100g DW, 387±0.7 mg TE/g DW and 5.9±0.5 µg GAE/g DW) as determined by ORAC, ABTS and DPPH assays, respectively. The acidic and basic hydrolysis yielded higher antioxidant capacities in the present study. It suggests that hydrolysis with alkaline and acidic play significant roles in liberating more phenolic acids and flavonoids and generating high antioxidant capacity in the extracts.

Pages 01-07
Year 2019
Issue 1
Volume 3

Download

Posted by Nurul

gws.02.2018.27.29

ABSTRACT

ALPHA-AMYLASE INHIBITORY ACTIVITY OF INHIBITOR PROTEINS IN DIFFERENT TYPES OF COMMERCIAL RICE

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Nur Syazila Ramli, Noor Hasniza Md Zin

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2018.27.29

The inhibition of α-amylase enzyme by the inhibitor proteins extracts of all the three types of commercial rice, white rice (WR), brown rice (BR) and glutinous rice (GR) were investigated. Among the three types of rice, the BR has significantly the highest concentration of the inhibitor protein (0.030 ± 0.002 mg/ml) compared to glutinous rice (0.006 ± 0.001 mg/ml) and white rice (0.005 ± 0.001 mg/ml). In term of the percentage of inhibition of the α-amylase enzyme of the Aspergillus oryzae, the brown rice shows the highest inhibition (61.22 %) among the three types of rice with the lowest maltose liberated. The acarbose (0.1 mg/ml) which was used as a positive control represent the highest inhibition of the α-amylase enzyme inhibitor among all of the tested samples. Even though inhibitor protein extract of BR is possessed lower ability to inhibit the α-amylase, yet it still can be one of the best option and alternative for the Diabetes Mellitus patients for their daily consumption compared to the WR and GR.

Pages 27-29
Year 2018
Issue 2
Volume 2

Download

Posted by Nurul

gws.02.2018.21.26

ABSTRACT

ISOLATION AND SCREENING OF BIOSURFACTANT-PRODUCING MARINE
BACTERIA FROM KUANTAN PORT, PAHANG, MALAYSIA

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Muhammad Isminhaziq Ismail, Nur Hafizah Azizan, Mardiana Mohd Ashaari

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2018.21.26

Biosurfactants play an important role in bioremediation of organic pollutants such as petroleum hydrocarbon. The unique properties of biosurfactants make them possible to be used in the remediation of hydrocarbon contaminated sites. Therefore, the existence of indigenous microorganisms that have the ability to consume petroleum hydrocarbon as carbon source and simultaneously produce biosurfactants in order to facilitate the hydrocarbon metabolism can be manipulated for bioremediation purposes. In this study, isolation and screening of potential biosurfactant-producing bacteria from two sampling points in Kuantan Port seawater were successfully done. Amongst the isolates, 4 out of 7 isolates from Point A were Gram negative bacteria and 2 out 5 isolates from Point B were Gram negative bacteria. The positive oxidase test resulted for all isolates from Point A and only B5 from Point B produced negative result. Catalase test conducted produced positive results on isolates from Point A (A3, A5, A6& A7) and Point B (B1, B2, B4 & B5).The highest percentage emulsification index measured belonged to isolate B4 and B5 which are 67%, thus make these isolates to be the most promising biosurfactant producers. Further identification by 16S rRNA gene found that isolates were closely related to Rhodococcus erythropolis (A1), Psedomonas stutzeri (A2), Pseudoalteromonas lipolytica (A3, A6 and B4), Vibrio brasiliensis (A4 and B2), Vibrio tubiashii (B1), Marinobacter salsuginis (A5), Labrenzia aggregate (A7), Marinococcus halophilus (B3) and Thalassospira xianmenensis(B5). Hence, through biosurfactant activities exhibited by isolates, B4 and B5 were the most potential isolates to produce biosurfactant. Therefore, these isolates can potentially be exploited to aid in bioremediation of petroleum hydrocarbon contaminated sites and would also be useful to enhance oil recovery in petroleum industry.

Pages 21-26
Year 2018
Issue 2
Volume 2

Download

Posted by Nurul

gws.02.2018.16.20

ABSTRACT

RESEARCH ON MENTAL HEALTH STATUS AND THE RELATIONSHIP BETWEEN SPIRITUAL BELIEF AND SELF – HARMONY

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Liu Chuanlei , Li Guomin , Han Yuanfei, Wu Guojun

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2018.16.20

According to the questionnaire survey of 500 graduate students on mental health, spiritual belief and self-harmony, through mathematical statistical analysis, it was found that :(1) overall, the psychological status of graduate students was unhealthy, and there were significant differences in some demographic variables; (2) self-flexibility has a significant positive predictive effect on political belief, nationalism, life pursuit and family pursuit; (3) the rigidity of ego has significant negative and positive predictive effect on nationalism and money pursuit respectively; (4) the disharmony between self and experience has a significant positive predictive effect on religious belief and god worship.

Pages 16-20
Year 2018
Issue 2
Volume 2

Download

Posted by Nurul

gws.02.2018.10.15

ABSTRACT

CYTOTOXIC EFFECTS AND RESPONSE SURFACE OPTIMIZATION OF SOLVENT EXTRACTION OF CRUDE EXTRACTS FROM Aquilaria subintegra UNINFECTED BRANCH

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Phirdaous Abbas, Yumi Z.H-Y Hashim, Hamzah Mohd Salleh

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2018.10.15

Agarwood plant has been used in traditional medicine to treat wide range of disorders aside from the main function of its fragrant wood in perfumery. In this present study, we are interested to maximize the yield of extract and investigate the cytotoxic/anti-cancer effects of uninfected branch extracts. Uninfected branch is readily available from agarwood plantation particularly during the early years before inoculation process that formed the fragrant wood (resin); and as such is seen as an economical raw material. Uninfected branch from Aquilaria subintegra was subjected to response surface methodology (RSM) – guided ethanolic extraction to achieve maximum yield while maintaining the biological activity. Cytotoxicity/anti-cancer assays including cell attachment assay, cell viability assay and sulforhodamine B (SRB) assay were carried out on the extracts. Run 11 with 12 hours extraction time, 50 °C temperature, 100 rpm and 60 ml extraction volume gave the highest yield of 0.2130 ± 0.036 g/g agarwood branch extract (ABE). Experimental extract from run 16 showed the most promising cytotoxic effects against MCF-7 with IC50 of 8 µg/ml. The study showed that ABE possess potential cytotoxic/anti-cancer activities against MCF-7 cells and further research is warranted to identify the bioactive compounds and mechanism of action.

Pages 10-15
Year 2018
Issue 2
Volume 2

Download

Posted by Nurul

gws.02.2018.04.09

ABSTRACT

DIVERSITY OF BIVALVES IN MANGROVE FOREST, TOK BALI KELANTAN, MALAYSIA

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Zaleha Kassim, Zuhairi Ahmad, Norshida Ismail

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2018.04.09

A study on the diversity and some ecological aspects that related to the abundance of infaunal bivalve species was done at Tok Bali mangrove, Kelantan, Malaysia. Samples and data collection was conducted during three different seasonal periods, on dry season (July), pre-monsoon (September) and monsoon (December) in the year 2005. Sampling stations were chosen in four mangrove forests which were encompassed with Rhizophora spp., Avicennia spp., Nypa fruticans and Mixed Mangrove. A collection of bivalve samples and sediment samples were done within 0.25 m2 quadrates and measurement of physico-chemical parameters were conducted using Hydrolab Quanta. Temperature, salinity and pH showed the normal mangrove value and decreased during monsoon, while dissolved oxygen show increasing during monsoon. Mean of grain size (ø) value ranged from 1.9 to 2.66 indicated that the sediment is fine sand. Mean TOM ranged from 0.67-1.45 g/g. A total of five (5) species of infaunal bivalves were observed, which were Polymesoda expansa, Marcia japonica, Gari ambigua, Pillsbryoconcha exilis and Donax faba. Diversity index H’ ranges from 0.72-1.27 and evenness index E’ ranged from 0.53-0.95 and richness index varied from 0.42-0.78. ANOVA tests showed that there were no significant differences for all biodiversity indices during dry, pre-monsoon and monsoon season (P>0.05). The results showed the low biodiversity of infaunal bivalves in the mangroves of Tok Bali. They could be more affected by the spatial factors rather than the monsoon. Long-term data collection is suggested to determine the seasonal pattern of their biodiversity and contribution to the mangrove ecosystem in the area.

Pages 04-09
Year 2018
Issue 2
Volume 2

Download

Posted by Nurul

gws.02.2018.01.03

ABSTRACT

RESEARCH ON DESIGN PRINCIPLES OF VISUAL IDENTITY IN CAMPUS ENVIRONMENT

Journal: Science Heritage Journal | Galeri Warisan Sains (GWS)
Author: Feng Qiao

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Doi: 10.26480/gws.02.2018.01.03

The study analyzes the current status of vision according to the environment of the campus, to understand the main problems and the reasons for its existence, combined with a large number of actual design cases, the use of field exploration and access methods of phenomenology, thinking of visual identity design to adapt to the campus environment, and further into the future for designers reference campus visual logo design principles.

Pages 01-03
Year 2018
Issue 2
Volume 2

Download

Posted by Nurul